Carboxymethylated Rhizoma alismatis Polysaccharides Regulate Calcium Oxalate Crystals Growth and Reduce the Regulated Crystals’ Cytotoxicity
نویسندگان
چکیده
Objective: This study explored the effects of polysaccharides (RAPD) extracted from traditional anti-stone Chinese medicine Rhizoma alismatis and their carboxymethylated derivatives (RAPs) on crystal phase, morphology, size calcium oxalate (CaOx). It also determined damaging ability regulated crystals human renal tubular epithelial cells (HK-2). Methods: RAPD carboxymethylation with a carboxyl group (–COOH) content 3.57% was carried out by chloroacetic acid solvent method. The –COOH in RAPs RAP concentration regulation CaOx growth were studied controlling variables. Cell experiments conducted to explore differences cytotoxicity RAP-regulated crystals. Results: contents RAPD, RAP1, RAP2, RAP3 3.57%, 7.79%, 10.84%, 15.33%, respectively. can inhibit monohydrate (COM) induce formation dihydrate (COD). When high, COD enhanced. In induced RAPs, high lower damage cells. particular, lowest. increased, gradually increased due reduced formed An interaction observed between crystals, number adsorbed positively correlated RAPs. Conclusions: reduce HK-2 regulating crystallization effectively reducing risk kidney stone formation. especially content, has potential be developed as novel green drug.
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ژورنال
عنوان ژورنال: Biomolecules
سال: 2023
ISSN: ['2218-273X']
DOI: https://doi.org/10.3390/biom13071044